A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. Series: Methods in Molecular Biology (Book 288) Hardcover: 436 pages Publisher: Humana Press; 2005 edition (September 1, 2004) Language: English ISBN-10: 1588292339 ISBN-13: 978-1588292339 Product Dimensions: 6.1 x 1 x 9.2 inches Link Download http://nitroflare.com/view/B3D1AF61A6B2701https://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1
