Methods in Molecular Biology Vol.288 Oligonucleotide Synthesis Methods and Applications

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    admin Thư Viện Sách Việt Staff Member Quản Trị Viên

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    A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
    • Series: Methods in Molecular Biology (Book 288)
    • Hardcover: 436 pages
    • Publisher: Humana Press; 2005 edition (September 1, 2004)
    • Language: English
    • ISBN-10: 1588292339
    • ISBN-13: 978-1588292339
    • Product Dimensions: 6.1 x 1 x 9.2 inches
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    Last edited: Nov 21, 2021

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