Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included. Series: Methods in Molecular Biology (Book 298) Hardcover: 262 pages Publisher: Humana Press; 2005 edition (February 28, 2005) Language: English ISBN-10: 1588293173 ISBN-13: 978-1588293176 Product Dimensions: 6.1 x 0.7 x 9.2 inches Link Download http://nitroflare.com/view/9C374D46CB6DDE4https://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1
