This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition. Series: Methods in Molecular Biology (Book 389) Hardcover: 268 pages Publisher: Humana Press; 2nd edition Language: English ISBN-10: 0205314570 ISBN-13: 978-1588294296 ASIN: 1588294293 Product Dimensions: 9 x 6.3 x 0.9 inches Link Download http://nitroflare.com/view/68723FA88E0AA67https://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1
