From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis. Coverage extends the potential of realtime as well as advocates simplifications of the probe technologies. It also presents a new simplified molecular beacon design, EasyBeacons, and demonstrates the utility in DNA methylation profiling. Series: Methods in Molecular Biology (Book 429) Hardcover: 266 pages Publisher: Humana Press; 2008 edition (April 3, 2008) Language: English ISBN-10: 1855675471 ISBN-13: 978-1588297006 ASIN: 1588297004 Product Dimensions: 6.1 x 0.7 x 9.2 inches Link Download http://nitroflare.com/view/AEDB019011A1182https://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1