In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Series: Methods in Molecular Biology (Book 630) Hardcover: 341 pages Publisher: Humana Press; 2nd ed. 2010 edition (March 22, 2010) Language: English ISBN-10: 1607616289 ISBN-13: 978-1607616283 Product Dimensions: 9.9 x 7.2 x 1 inches Link Download http://nitroflare.com/view/0F08EDB5E773E3Fhttps://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1
