Methods in Molecular Biology Vol.951 Mass Spectrometry of Glycoproteins Methods and Protocols

Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics. These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

• Series: Methods in Molecular Biology (Book 951)
• Hardcover: 339 pages
• Publisher: Humana Press;
  
• Language: English
• ISBN-10: 1627031456
• ISBN-13: 978-1627031455
• Product Dimensions: 10.3 x 7.2 x 1 inches

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