Methods in Molecular Medicine Vol.50 Colorectal Cancer Methods and Protocols

Colorectal cancer is a common and highly lethal cancer once spread whose treatment is improving as we gain a better understanding of its development. In Colorectal Cancer: Methods and Protocols, Steven M. Powell, MD, has assembled a hands-on collection of cutting-edge molecular methods for studying this solid tumor. These readily reproducible techniques range from gross microdissection of specimens to specific molecular analyses. Here both the novice and experienced researcher will find productive chromosomal analyses, including comparative genomic hybridization and FISH assays, and revealing gene expression analyses, including immunohistochemical assays, Western blotting, and microarray assays. Also presented are mutational analyses of colon tumors and blood samples to determine whether specific patients have inherited a significant predisposition to colorectal cancer. Each method is described in step-by-step detail by an established expert to ensure robust experimental results, and all are accompanied by detailed notes on pitfalls to avoid.

• Series: Methods in Molecular Medicine (Book 50)
• Paperback: 294 pages
• Publisher: Humana Press
• Language: English
• ISBN-10: 1617371793
• ISBN-13: 978-1617371790
• Product Dimensions: 6 x 0.7 x 9 inches

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